4GAW
Crystal structure of active human granzyme H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 170.369, 367.070, 61.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.800 - 3.000 |
| R-factor | 0.27249 |
| Rwork | 0.271 |
| R-free | 0.30593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tk9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.867 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | COMO |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.314 |
| Number of reflections | 78537 | |
| <I/σ(I)> | 19.7 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M Li2SO4, 0.1M Bicine (pH8.5), 25% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
