4GAI
Crystal structure of EBI-005, a chimera of human IL-1beta and IL-1Ra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.392, 66.910, 216.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.490 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.342 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 3.210 | 1.490 |
| Rmerge | 0.060 | 0.041 | 0.582 |
| Number of reflections | 51907 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 99.8 | 98.4 | 99.4 |
| Redundancy | 5.7 | 5.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25% PEG 3350, 200 mM MgCl2, 100 mM HEPES, pH 7.5, 0.5% w/v n-Dodecy- -D-maltoside (DDM), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
