4GA6
Crystal structure of AMP phosphorylase C-terminal deletion mutant in complex with substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.177, 87.915, 105.549 |
| Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.210 |
| Rwork | 0.214 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ga5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 46007 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 98.9 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1M phosphate-citrate, 20% (w/v) PEG 1000, 50mM Li2SO4, 0.2M guanidine-HCl, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
