4G9Z

Lassa nucleoprotein with dsRNA reveals novel mechanism for immune suppression

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	196
Detector technology	PIXEL
Collection date	2011-06-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.8726
Spacegroup name	P 41 21 2
Unit cell lengths	46.744, 46.744, 217.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.330 - 2.030
R-factor	0.20496
Rwork	0.203
R-free	0.24041
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.220
High resolution limit [Å]	1.990
Rmerge	0.118
Number of reflections	15723
<I/σ(I)>	6.8
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	293	18% PEG MME 5000, 0.1M Na-citrate, pH4.5, 2.4% PEG MME350, EVAPORATION, temperature 293K