4G8K

Intact sensor domain of human RNase L in the inactive signaling state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	77
Detector technology	CCD
Collection date	2011-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.072
Spacegroup name	P 21 21 21
Unit cell lengths	62.800, 63.000, 241.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.375 - 2.400
R-factor	0.2189
Rwork	0.217
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wdy
RMSD bond length	0.002
RMSD bond angle	0.593
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.375	60.000	2.500
High resolution limit [Å]	2.400	10.000	2.400
Number of reflections	38136
Completeness [%]	99.1	98.2	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	50 mM potassium acetate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, temperature 293K