4G8K
Intact sensor domain of human RNase L in the inactive signaling state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.072 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.800, 63.000, 241.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.375 - 2.400 |
R-factor | 0.2189 |
Rwork | 0.217 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wdy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.593 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.375 | 60.000 | 2.500 |
High resolution limit [Å] | 2.400 | 10.000 | 2.400 |
Number of reflections | 38136 | ||
Completeness [%] | 99.1 | 98.2 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 50 mM potassium acetate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, temperature 293K |