4G7N
The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-01 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.00890 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.773, 135.580, 46.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.776 - 2.300 |
| R-factor | 0.1908 |
| Rwork | 0.185 |
| R-free | 0.25540 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22051 | |
| <I/σ(I)> | 16.1 | 3.8 |
| Completeness [%] | 89.3 | 84.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | an equal volume of 7.2 mg/ml Plk4 PB1-PB2 and well solution containing 1.2 M Li2SO4, 100 mM Hepes, pH 7.5 were mixed together and equilibrated over 1 ml of well solution., VAPOR DIFFUSION, HANGING DROP, temperature 300K |
