4G5S
Structure of LGN GL3/Galphai3 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 209.680, 209.680, 235.482 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.626 - 3.620 |
R-factor | 0.2096 |
Rwork | 0.207 |
R-free | 0.25290 |
RMSD bond length | 0.017 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.660 |
High resolution limit [Å] | 3.600 | 9.750 | 3.600 |
Rmerge | 0.073 | 0.035 | 0.396 |
Number of reflections | 35082 | ||
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.7 | 95.2 | 100 |
Redundancy | 9.2 | 7.6 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |