4G5R
Structure of LGN GL4/Galphai3 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 209.550, 209.550, 237.211 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.044 - 3.481 |
R-factor | 0.2113 |
Rwork | 0.209 |
R-free | 0.25280 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.884 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.480 | 3.500 |
Rmerge | 0.110 | 0.757 |
Number of reflections | 39267 | |
<I/σ(I)> | 14.7 | 1.8 |
Redundancy | 4.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |