4G4N

MutM containing M77A mutation bound to undamaged DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.188, 93.541, 104.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.685 - 1.850
R-factor	0.1703
Rwork	0.169
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.685	1.900
High resolution limit [Å]	1.850	1.850
Number of reflections	37757
Completeness [%]		91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 8K, sodium cacodylate, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K