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4G3D

Crystal structure of human NF-kappaB inducing kinase (NIK)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL11-1
Synchrotron siteSSRL
BeamlineBL11-1
Temperature [K]93
Detector technologyCCD
Collection date2008-05-28
DetectorMARMOSAIC 325 mm CCD
Wavelength(s)0.97945
Spacegroup nameP 21 21 21
Unit cell lengths45.746, 145.851, 230.818
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.570 - 2.900
R-factor0.20601
Rwork0.201
R-free0.26646
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.005
RMSD bond angle1.039
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.6.0117)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.000
High resolution limit [Å]2.9002.900
Number of reflections35489
<I/σ(I)>21.83.6
Completeness [%]100.0100
Redundancy7.37.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.52771:1 protein and well solution of 100 mM imidazole (pH 6.5), 1 M sodium acetate tri-hydrate, 7.5% 1,6-hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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