4G33
Crystal Structure of a Phospholipid-Lipoxygenase Complex from Pseudomonas aeruginosa at 2.0 A (C2221)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.850, 97.276, 157.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.030 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g32 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.030 |
| Number of reflections | 42120 | |
| <I/σ(I)> | 9 | 2.2 |
| Completeness [%] | 99.2 | 94.9 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 3350, 0.2M MgCl2, 0.1M TRIS pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
