4G1N
PKM2 in complex with an activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.118, 94.141, 146.149 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 40.240 - 2.300 |
| Rwork | 0.211 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.125 |
| Number of reflections | 94063 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 99.7 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.8 | 291 | 0.1 M Imidazole pH 7.8, 20 %(w/v) PEG 6000, 15 mg/ml PKM2, EVAPORATION, temperature 291K |
