4G0R
Structural characterization of H-1 Parvovirus: comparison of infectious virions to replication defective particles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2010-06-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9186 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 255.400, 350.400, 271.600 |
Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
Rwork | 0.216 |
R-free | 0.21690 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.365 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 1127217 | |
<I/σ(I)> | 6.8 | 2.4 |
Completeness [%] | 93.1 | 94.7 |
Redundancy | 2.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | The reservoir solution contained 1-3% (w/v) polyethylene glycol 8000 (PEG 8000), 150 mM NaCl, and 8mM CaCl2.2H2O as precipitants in 10 mM Tris. The drops were prepared by mixing equivolume of virus solution (10mg/ml) in Tris-HCl Buffer (10 mM Tris-HCl, 150 mM NaCl, 8mM CaCl2.2H2O) with reservoir solution, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |