4FXW
Structure of phosphorylated SF1 complex with U2AF65-UHM domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-19 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9219, 0.9794, 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.800, 116.100, 130.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.560 - 2.290 |
R-factor | 0.2513 |
Rwork | 0.248 |
R-free | 0.28070 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.656 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 2.290 | 6.100 |
Rmerge | 0.062 | 0.039 |
Number of reflections | 50161 | |
<I/σ(I)> | 28.9 | |
Completeness [%] | 99.6 | 95.5 |
Redundancy | 4.4 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.2 M Lithium sulfate, 20% PEG 3350 and 0.1 M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |