4FWJ
Native structure of LSD2/AOF1/KDM1b in spacegroup of I222 at 2.9A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2011-06-24 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 144.322, 170.933, 202.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.18813 |
| Rwork | 0.186 |
| R-free | 0.22233 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hko |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.281 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.113 | 0.443 |
| Number of reflections | 55566 | |
| <I/σ(I)> | 15.7 | 2.9 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
