4FUB
Crystal Structure of the Urokinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 160 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.100, 52.400, 80.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.740 - 1.900 |
R-factor | 0.1567 |
Rwork | 0.155 |
R-free | 0.18670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.640 |
High resolution limit [Å] | 1.600 | 3.940 | 1.600 |
Rmerge | 0.122 | 0.082 | 0.661 |
<I/σ(I)> | 5 | ||
Completeness [%] | 86.3 | 92.5 | 44.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.15 M Li2SO4, 20% polyethylene glycol MW 4000 in succinate buffer, pH 4.8-6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |