Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | H 3 |
Unit cell lengths | 326.040, 326.040, 770.150 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 3.200 |
Rwork | 0.259 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Wild-type FHV coordinates |
RMSD bond length | 0.010 |
RMSD bond angle | 1.493 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.103 | 0.583 |
Number of reflections | 463709 | |
<I/σ(I)> | 11 | 1.6 |
Completeness [%] | 92.3 | 80.2 |
Redundancy | 3.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 295 | 5-6% PEG 8K, 0.15M LITHIUM SULFATE, 0.1M HEPES AND 0.01M CALCIUM CHLORIDE, pH 7.0, vapor diffusion, temperature 295K |