4FSS

Crystal structure of a RAS p21 protein activator (RASA1) from Homo sapiens at 2.25 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97968,0.97901
Spacegroup name	P 61 2 2
Unit cell lengths	82.220, 82.220, 157.212
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.449 - 2.250
R-factor	0.2144
Rwork	0.213
R-free	0.23740
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.449	29.449	2.330
High resolution limit [Å]	2.250	4.830	2.250
Rmerge	0.223	0.015	0.015
Number of reflections	15649	2869	2784
<I/σ(I)>	7.78	18.7	1.4
Completeness [%]	99.7	99.3	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	20.00% Glycerol, 1.600M NH4H2PO4, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K