4FRW
Crystal structure of human nectin-4 extracellular fragment D1-D2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.971, 142.809, 341.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.25898 |
| Rwork | 0.257 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3alp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.940 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.850 | 3.500 |
| Number of reflections | 26677 | ||
| <I/σ(I)> | 7.8 | 2.4 | |
| Completeness [%] | 99.4 | 100 | 100 |
| Redundancy | 5.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 3 M sodium chloride, 0.1 M Bis-Tris, pH 5.5, cryoprotectant: 30% w/v D-Trehalose, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
