Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.259, 79.259, 129.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.980 - 2.800 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yv8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.096 | 0.385 |
Number of reflections | 10378 | |
<I/σ(I)> | 9.3 | 3.3 |
Completeness [%] | 95.6 | 92.8 |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 10% PEG 20000, 0.1M Bicine, 2% Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |