4FO9
Crystal structure of the E3 SUMO Ligase PIAS2
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28295 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.288, 170.140, 57.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.790 - 2.390 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.25680 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (refmac_5.7.0027) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.390 | 6.510 | 2.400 |
| Rmerge | 0.087 | 0.043 | 0.873 |
| Number of reflections | 17845 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 99.8 | 96.3 | 100 |
| Redundancy | 7.7 | 6.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.7 M KH2PO4/NaH2PO4, pH6.5, vapor diffusion hanging drop, temperature 291K |
