4FNL

Crystal structure of broadly neutralizing antibody C05

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 41 21 2
Unit cell lengths	160.130, 160.130, 135.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.219 - 2.297
R-factor	0.2138
Rwork	0.212
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1AQK AND 1VGE
RMSD bond length	0.003
RMSD bond angle	0.993
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.297	2.297
Number of reflections	78741
<I/σ(I)>	20.4	2.2
Completeness [%]	99.9	99.6
Redundancy	13.2	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	277	1.8 M ammonium sulfate, 100 mM sodium acetate, pH 5.4, 50 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K