4FMK
Crystal structure of mouse nectin-2 extracellular fragment D1-D2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 68.666, 68.666, 159.605 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.560 |
R-factor | 0.22332 |
Rwork | 0.221 |
R-free | 0.26755 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3alp |
RMSD bond length | 0.016 |
RMSD bond angle | 1.667 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.450 | 2.630 |
High resolution limit [Å] | 2.540 | 3.200 | 2.540 |
Number of reflections | 14118 | ||
<I/σ(I)> | 20.5 | 3.9 | |
Completeness [%] | 99.4 | 99.9 | 99.4 |
Redundancy | 6.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 6% w/v PEG6000, 0.05 M cadmium sulfate, 0.2 M sodium acetate, 0.1 M MES, pH 6.0, cryoprotectant: 15% 2R,3R-butane-di-ol, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |