4FM0
Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.980110 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.610, 114.720, 128.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.320 - 3.120 |
R-factor | 0.1788 |
Rwork | 0.177 |
R-free | 0.20820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4flw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 3.290 |
High resolution limit [Å] | 3.100 | 3.120 |
Rmerge | 0.131 | 0.660 |
Number of reflections | 18760 | |
<I/σ(I)> | 8.4 | 2.4 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 3.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-12% PEG 20000, 100 mM MES pH 6.5, seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K |