4FL2
Structural and Biophysical Characterization of the Syk Activation Switch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-20 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.562, 70.722, 76.369 |
| Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
| Resolution | 46.080 - 2.190 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.080 | 2.330 |
| High resolution limit [Å] | 2.190 | 2.200 |
| <I/σ(I)> | 2.78 | |
| Completeness [%] | 99.0 | 94.5 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12% PEG 3350, 100 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
