4FKB
An Organic solvent tolerant lipase 42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-02-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.75 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.824, 81.001, 99.529 |
Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.220 |
R-factor | 0.12894 |
Rwork | 0.127 |
R-free | 0.16458 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.025 |
RMSD bond angle | 2.308 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.252 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.056 | |
Number of reflections | 275120 | |
Completeness [%] | 93.7 | 93.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 8 | 293.15 | Counter-diffusion method without mixing the protein and precipitant, pH 8, LIQUID DIFFUSION, temperature 293.15K |