4FKA
High resolution structure of the manganese derivative of insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.700, 81.700, 33.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.280 - 1.080 |
| R-factor | 0.16114 |
| Rwork | 0.159 |
| R-free | 0.19447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3exx |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.420 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.480 |
| High resolution limit [Å] | 1.000 |
| Rmerge | 0.089 |
| Number of reflections | 43875 |
| <I/σ(I)> | 11 |
| Completeness [%] | 96.7 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | The protein solution consisted of 7.5 mg mL-1 of zinc-free insulin in 0.02 mol L-1 hydrochloric acid. The reservoir solution was at pH 6.4 containing 1mM sodium citrate, volume fraction of aceton 10 % ,9 mmol L-1 manganese(II) sulphate monohydrate and redistilled water, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
