4FIW

X-ray crystal structure of Corynebacterium glutamicum Nrdh-redoxin at 1.5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	61.770, 61.770, 77.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.675 - 1.501
R-factor	0.1931
Rwork	0.191
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1R74 1h75 3IC4 and 1ABA
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.675	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	14472
Completeness [%]	98.3	94.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	12% glycerol, 0.1M Tris pH 8.5, 1.8M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K