4FII
Catalytic domain of human PAK4 with RPKPLVDP peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97550 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.557, 61.557, 181.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.20347 |
| Rwork | 0.201 |
| R-free | 0.24038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cdz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.114 | 0.778 |
| <I/σ(I)> | 25.4 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M Tris-HCl, 1.5M Na acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
