4FI1
Crystal structure of scCK2 alpha in complex with ATP
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.773, 69.773, 168.514 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.430 - 2.090 |
R-factor | 0.2063 |
Rwork | 0.204 |
R-free | 0.24317 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.188 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.430 | 2.149 |
High resolution limit [Å] | 2.090 | 2.095 |
Number of reflections | 29501 | |
Completeness [%] | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 287 | 4 mM magnesium chloride, 200 mM ammonium sulfate, 100 mM MES (pH 6.2), 20% w/v polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 287K |