4FI1
Crystal structure of scCK2 alpha in complex with ATP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.773, 69.773, 168.514 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.430 - 2.090 |
| R-factor | 0.2063 |
| Rwork | 0.204 |
| R-free | 0.24317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.188 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.430 | 2.149 |
| High resolution limit [Å] | 2.090 | 2.095 |
| Number of reflections | 29501 | |
| Completeness [%] | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 287 | 4 mM magnesium chloride, 200 mM ammonium sulfate, 100 mM MES (pH 6.2), 20% w/v polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
