4FH5
Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgUTP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-03-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.580, 62.860, 111.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.280 - 2.300 |
R-factor | 0.20393 |
Rwork | 0.202 |
R-free | 0.24447 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fh3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.185 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.600 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 17243 | |
<I/σ(I)> | 14.5 | 4.4 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 100 mM CHES, pH 9.5, 5-10% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |