4FH2
Structure of s70c beta-lactamase bound to sulbactam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-13 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.971, 55.585, 84.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.760 - 1.440 |
R-factor | 0.2064 |
Rwork | 0.206 |
R-free | 0.21996 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.223 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.5.0110) |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.760 | 1.550 |
High resolution limit [Å] | 1.440 | 1.450 |
Rmerge | 0.053 | 0.655 |
Number of reflections | 35770 | |
<I/σ(I)> | 30.2 | 2.05 |
Completeness [%] | 96.2 | 95.8 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 21-30%PEG6k, 0.1M hepes, 0.56mM cymal-6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |