4FGU
Crystal structure of prolegumain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 185.100, 185.100, 173.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.630 - 3.900 |
R-factor | 0.28648 |
Rwork | 0.286 |
R-free | 0.29963 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.169 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.800 | 4.110 |
High resolution limit [Å] | 3.900 | 3.900 |
Number of reflections | 10515 | |
Completeness [%] | 99.8 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 35 % 2-ethoxyethanol, 0.1 M cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |