4FFZ
Crystal Structure of DENV1-E111 fab fragment bound to DENV-1 DIII (Western Pacific-74 strain).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.007 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.865, 52.013, 136.402 |
Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
Resolution | 43.448 - 3.800 |
R-factor | 0.2375 |
Rwork | 0.237 |
R-free | 0.27760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aeh 4ffy |
RMSD bond length | 0.003 |
RMSD bond angle | 0.859 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.7.3) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.970 |
High resolution limit [Å] | 3.800 | 7.590 | 3.800 |
Rmerge | 0.120 | 0.056 | 0.514 |
Number of reflections | 11029 | ||
<I/σ(I)> | 9.74 | 1.91 | |
Completeness [%] | 98.0 | 95.5 | 98.6 |
Redundancy | 4.1 | 3.8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 6000, 0.1M MES, 1% glycerol., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |