4FBP

CONFORMATIONAL TRANSITION OF FRUCTOSE-1,6-BISPHOSPHATASE: STRUCTURE COMPARISON BETWEEN THE AMP COMPLEX (T FORM) AND THE FRUCTOSE 6-PHOSPHATE COMPLEX (R FORM)

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	78.500, 88.500, 211.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.500
R-factor	0.169 *
Rwork	0.169
RMSD bond length	0.013
RMSD bond angle	2.990
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.064 *
Total number of observations	179560 *
Number of reflections	47359 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	buttom	AMP	1 (mM)
2	1	buttom	protein	12.5 (mg/ml)
3	1	reservoir	tris aminomethane	20 (mM)
4	1	reservoir	EDTA	0.1 (mM)
5	1	reservoir		5 (mM)
6	1	reservoir	beta-mercaptoethanol	12 (%(w/v))
7	1	reservoir	PRG3350	12 (%(v/v))