4F9G
Crystal structure of STING complex with Cyclic di-GMP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.027, 74.200, 60.013 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 38.298 - 2.950 |
| R-factor | 0.2099 |
| Rwork | 0.205 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f9e |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.534 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
| Rmerge | 0.066 | 0.035 | 0.485 |
| Number of reflections | 11107 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 98.9 | 91.7 | 100 |
| Redundancy | 5.8 | 5.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 12-15% PEG8000, 0.08 M sodium cacodylate pH 6.3-6.5, 0.16 M calcium acetate and 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
