4F8K
Molecular analysis of the interaction between the prostacyclin receptor and the first PDZ domain of PDZK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.271, 68.477, 40.124 |
Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
Resolution | 40.100 - 1.700 |
R-factor | 0.18611 |
Rwork | 0.184 |
R-free | 0.21914 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngh |
RMSD bond length | 0.013 |
RMSD bond angle | 1.591 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.100 |
High resolution limit [Å] | 1.700 |
Number of reflections | 23390 |
Completeness [%] | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES pH6.5, 30% (w/v) PEG 300, VAPOR DIFFUSION, SITTING DROP, temperature 291K |