4F8K
Molecular analysis of the interaction between the prostacyclin receptor and the first PDZ domain of PDZK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.271, 68.477, 40.124 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 40.100 - 1.700 |
| R-factor | 0.18611 |
| Rwork | 0.184 |
| R-free | 0.21914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.100 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 23390 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES pH6.5, 30% (w/v) PEG 300, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
