4F8E

Crystal structure of human PRS1 D52H mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-01
Detector	RAYONIX MX-325
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	170.310, 170.310, 61.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.270
Rwork	0.178
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.270	2.270
Number of reflections	30931
Completeness [%]		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.1	295	pH 4.1, VAPOR DIFFUSION, temperature 295K