4F5Y
Crystal structure of human STING CTD complex with C-di-GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97891 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.145, 81.438, 91.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.836 - 2.396 |
R-factor | 0.2146 |
Rwork | 0.211 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.195 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.396 | 5.170 | 2.400 |
Rmerge | 0.089 | 0.040 | 0.501 |
Number of reflections | 22031 | ||
<I/σ(I)> | 25.05 | 54.96 | 3.89 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 7.2 | 6.9 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15% PEG 8000, 0.08M sodium cacodylate pH6.5, 0.16M calcium acetate and 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |