4F5T
Crystal Structure of Equine Serum Albumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-31 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8123 |
Spacegroup name | P 61 |
Unit cell lengths | 96.573, 96.573, 143.802 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.320 |
R-factor | 0.19913 |
Rwork | 0.197 |
R-free | 0.25852 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f5s |
RMSD bond length | 0.022 |
RMSD bond angle | 2.093 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.087 | 0.521 |
Number of reflections | 31502 | |
<I/σ(I)> | 17.4 | 1.93 |
Completeness [%] | 94.6 | 68.7 |
Redundancy | 6.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.7 M ammonium sulfate, 0.1M acetate buffer pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |