4F4O
Structure of the Haptoglobin-Haemoglobin Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 .0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.880, 197.780, 322.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.2116 |
Rwork | 0.211 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qpw 2qyo |
RMSD bond length | 0.012 |
RMSD bond angle | 1.620 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 5.000 | 3.000 |
High resolution limit [Å] | 2.900 | 4.000 | 2.900 |
Rmerge | 0.110 | 0.067 | 0.724 |
Number of reflections | 103264 | ||
<I/σ(I)> | 13.7 | 21.53 | 2.03 |
Completeness [%] | 99.0 | 99.5 | 94.6 |
Redundancy | 4.37 | 4.32 | 4.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 18% PEG3350 10% Jeffamine M-600 200 mM Ammonium Citrate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |