4F4K
DNA Polymerase I Large Fragment Complex 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2011-11-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.810, 109.220, 150.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.062 - 1.600 |
| R-factor | 0.1719 |
| Rwork | 0.171 |
| R-free | 0.19410 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2hvi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.353 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (dev_1026) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 4.780 | 1.600 |
| Rmerge | 0.045 | 0.027 | 0.504 |
| Number of reflections | 187320 | 7793 | 30461 |
| <I/σ(I)> | 21.29 | 52.85 | 3.61 |
| Completeness [%] | 92.1 | 96.6 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 53% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MnSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
