4F3O
DNA Polymerase I Large Fragment Complex 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Detector technology | CCD |
Collection date | 2012-03-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.830, 109.330, 150.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.565 - 1.570 |
R-factor | 0.1803 |
Rwork | 0.179 |
R-free | 0.20360 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED |
RMSD bond length | 0.010 |
RMSD bond angle | 1.357 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (dev_1026) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.660 |
High resolution limit [Å] | 1.570 | 4.690 | 1.570 |
Rmerge | 0.048 | 0.027 | 0.462 |
Number of reflections | 199455 | 8483 | 32723 |
<I/σ(I)> | 26.3 | 69.29 | 3.75 |
Completeness [%] | 92.9 | 99.6 | 99.9 |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 49%-51% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MnSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |