4F33
Crystal Structure of therapeutic antibody MORAb-009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 141.311, 141.311, 281.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.818 - 1.749 |
| R-factor | 0.2083 |
| Rwork | 0.208 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Selected by BALBES automatically |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.237 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: dev_965)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.749 | 1.749 |
| <I/σ(I)> | 15.9 | 1.4 |
| Completeness [%] | 92.0 | 70.7 |
| Redundancy | 5.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 294 | 26% Polyethylene glycol 400, 10% 2-propanol, 100 mM Sodium Citrate pH 5.0, VAPOR DIFFUSION, temperature 294K |
