4F2D
Crystal Structure of Escherichia coli L-arabinose Isomerase (ECAI) complexed with Ribitol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.472, 116.472, 214.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.21401 |
| Rwork | 0.212 |
| R-free | 0.25505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ajt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 85584 |
| <I/σ(I)> | 22.3 |
| Completeness [%] | 99.5 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, TRISODIUM CITRATE DIHYDRATE, Ribitol, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, TRISODIUM CITRATE DIHYDRATE, Ribitol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
