4F23
Influenza A virus hemagglutinin H16 HA0 structure with an alpha-helix conformation in the cleavage site: a potential drug target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Collection date | 2012-05-03 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.332, 240.944, 69.673 |
| Unit cell angles | 90.00, 119.79, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.16143 |
| Rwork | 0.160 |
| R-free | 0.19222 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.500 | 1.770 |
| High resolution limit [Å] | 1.700 | 1.710 |
| Number of reflections | 200026 | |
| Completeness [%] | 98.5 | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Imidazole, 20% (v/v) Jeffamine ED-2001, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
