4F1W
Crystal structure of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Salmonella enterica with Adenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.050, 68.120, 89.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.950 - 1.360 |
| R-factor | 0.11455 |
| Rwork | 0.113 |
| R-free | 0.14597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o4v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.435 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.240 | 1.430 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.112 | |
| Number of reflections | 87459 | |
| <I/σ(I)> | 12.9 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11.5 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.0M (NH4)2HPO4, 0.1M Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
