4F1I
Crystal structure of SeMet TDP2 from Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2011-09-09 |
| Detector | NOIR-1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.685, 100.685, 121.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.185 - 2.500 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.22880 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.878 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.126 | 0.828 |
| Number of reflections | 23342 | |
| <I/σ(I)> | 8.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | PEG 6.000, 0.3M ammonium chloride, and 0.1M MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
