4F1E

The Crystal Structure of a Human MitoNEET mutant with Asp 67 replaced by a Gly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	56.396, 110.855, 207.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.540 - 2.400
R-factor	0.195
Rwork	0.193
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2QH7
RMSD bond length	0.010
RMSD bond angle	1.140
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.540		2.490
High resolution limit [Å]	2.400	5.140	2.400
Rmerge	0.069	0.049	0.754
Number of reflections	51597	5584	5070
<I/σ(I)>	15.34	33.51	1.87
Completeness [%]	99.4	99.5	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1