4F0X
Crystal structure of human Malonyl-CoA Decarboxylase (Peroxisomal Isoform)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2011-06-16 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 80.420, 103.310, 134.240 |
Unit cell angles | 95.32, 90.22, 94.46 |
Refinement procedure
Resolution | 66.800 - 3.290 |
R-factor | 0.2456 |
Rwork | 0.244 |
R-free | 0.26670 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXS |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.820 | 3.470 |
High resolution limit [Å] | 3.290 | 3.290 |
Number of reflections | 63973 | |
<I/σ(I)> | 4.9 | 2 |
Completeness [%] | 97.7 | 97.7 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 16% PEG 2000 MME 0.1M AcNa 10mM CitNa, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |